Nicotine production occurs entirely within the tobacco roots, and is transported to the leaves (for protection from herbivores). Various plant stressors tend to increase nicotine production. Since I simply apply 10-10-10 fertilizer (at the appropriate rate for tomatoes) only once, in the week or two prior to transplanting to the field, and never add more fertilizer during the growing season [the exception being the rare event of drenching rains within the first two weeks after transplanting, in which case I re-apply the fertilizer at ½ rate], I don't have a clear sense of what impact repeated fertilizer has on nicotine production during the growing season. Also, I have no experience with the various supplements that you have applied.
In the commercial tobacco regions in the US, the fertilizer schedule is similar to what I usually do. For cost reasons at the commercial scale, the N-P-K ratios are optimized for tobacco, specifically to minimize cost. At the home scale, the cost differences between 10-10-10 and tobacco-specific N-P-K ratios are insignificant.
My suggestion for your next growing season is to fertilize once, prior to transplant, for most of the crop. Then, as a control comparison, plant a smaller portion using the same fertilizer routine that you used for 2026. Compare the results. You get to be the laboratory.
Bob
