Glad to see I'm not the only one using tobacco plants for their landscapingAnd if we make a "bigger" tobacco, then I may need a taller step ladder (with hand rails).
Garden variety allo-tetraploid tobacco (2015).
Bob
Oldfella
Well-Known Member
Why not just lean out of the window? It's safer than the ladder.And if we make a "bigger" tobacco, then I may need a taller step ladder (with hand rails).
Garden variety allo-tetraploid tobacco (2015).
Bob
Oldfella
Tutu
Well-Known Member
It’s been a long time since I started this thread and it kept me busy for a good while. Bob, thanks for opening the thread once again. The idea of making a cross had me very excited for a while but i was too optimistic. At the time i thought that by making a large number of attempts it would sooner or later work out. I guess that i turned from optimist to realist and will now go down a different path.
I have ordered half a laboratory setup. Microscope, still air box, Petri dishes, scalpels, tweezers and a whole bunch of other items.
The plan is as follows. I will make a sexual cross of N. rustica x N. tabacum. Rather than waiting for sheer luck (all previous attempts) i will harvest immature seeds early. I will then grow them in vitro in an MS medium with sugar and agar.
I am sure i will still need to make many attempts before I’ll succeed, but in theory this has a much higher success rate compared to what I’ve been doing before.
After growing various samples in vitro, hopefully i will be able to bring some specimen to adulthood. The next step would be to then get offspring from the plant which will involve some other steps. Earlier in the thread you guys mentioned using cholchicine for doubling chromosomes.
My plan is to get my hands on another herbicide for doing that, Oryzalin.
We’re still far away from those later stages. For now I’m excited for planning the setup of my provisional lab and which crosses i will be attempting. My main goal would be to cross CT Broadleaf as the paternal line and probably go with Aztec, Istela Puebla and Limonka as the maternal lines.
Stay tuned!
I have ordered half a laboratory setup. Microscope, still air box, Petri dishes, scalpels, tweezers and a whole bunch of other items.
The plan is as follows. I will make a sexual cross of N. rustica x N. tabacum. Rather than waiting for sheer luck (all previous attempts) i will harvest immature seeds early. I will then grow them in vitro in an MS medium with sugar and agar.
I am sure i will still need to make many attempts before I’ll succeed, but in theory this has a much higher success rate compared to what I’ve been doing before.
After growing various samples in vitro, hopefully i will be able to bring some specimen to adulthood. The next step would be to then get offspring from the plant which will involve some other steps. Earlier in the thread you guys mentioned using cholchicine for doubling chromosomes.
My plan is to get my hands on another herbicide for doing that, Oryzalin.
We’re still far away from those later stages. For now I’m excited for planning the setup of my provisional lab and which crosses i will be attempting. My main goal would be to cross CT Broadleaf as the paternal line and probably go with Aztec, Istela Puebla and Limonka as the maternal lines.
Stay tuned!
