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US Nicotiana Germplasm Collection 2013 Nursery

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skychaser

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I know he buys some seed from ProfiGen in Brazil. Also from Cross Creek, and from me. Probably others too.
 

Mad Oshea

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I trust the seed I get from the members here that have been here. I however never used pelleted seed. I have got some seed from the tobacco seed co. Can I trust the strains from them as well?
 

Knucklehead

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I trust the seed I get from the members here that have been here. I however never used pelleted seed. I have got some seed from the tobacco seed co. Can I trust the strains from them as well?

He has some made up names for his seed. You may have to email him to find out what variety he's using for "Burley Original" (TN90 last year) and "Virginia Gold #1" (K326 last year), "Virginia Gold #2" (a hybrid last year), "Virginia Gold #3" (another hybrid last year), etc. I don't know if those change with the availability of seed each year or not. Best to email and make sure. If Sky has the seed I need, that's where I'll buy. You can trust Sky's seed for sure and they have the correct varietal name.
 

Mad Oshea

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I have some of Sky's seed in this years grow. It is listed as Sky at the end of Type.The seed I got from You ,DGBAMA and ect. is tisted above the list. I will go with sky on that as well. Thanks. The only one not listed was Lattaque. Only one is up now from three.RC Old stock.
 

skychaser

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Such high praise. I really hope it grows now. lol

Jack (thetobaccoseed owner) had a hybrid Virginia Gold he was selling as Early VG a couple years ago that was CC27 from Cross Creek Seed. And another hybrid he just called Hybrid VG, which was actually PVH2306 from ProfiGen. They were both fantastic plants! I kind of hate to say it, but they kicked the heirloom VG types ass in overall production, leaf size and ease of curing.
 

Mad Oshea

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I love VG. All variaties. Mix is how they are used in cigs. only on My part. A smooth kick to the mix.LOL The Va 116 is a winner as well as the Pen Red. Thanks. You'v got to try the Bursa,Silver River and Lonnies Havanah that BGBAMA sent to Me. The seed came out of the shoot like a rocket-- Can't wait to see the out come. What a year this will be----------------
 

JessicaNicot

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That is true but remember Rickard's , Clays and Workman are now importing most of their seeds from Argentina and other countries . What may be illegal here may be legal over there .

I can't say about the workings of Rickards, but I do know that Gold Leaf grows all of their own and I think only locally in SC (and Cross Creek may also grow all of their own). The thing about US varieties are that variety developers (like NCSU, UK, etc) often have certified seed agreements with specific companies for the exclusive production and sale of XYZ variety seed. I imagine the same might be true for private tobacco companies (altho they are trending towards consolidation with less players actually developing new varieties).

I talked to my boss some about this yesterday and he said that tobacco was moving away from PVP (plant variety protection; like a patent) because of the introduction of male sterile varieties, but if seed resale starts to become an issue I forsee a return to pvp and harsh litigation (on behalf of both seed companies and variety developers).

given that, there are still lots of good things out there that are off pvp, like K326 and TN90. and altho there may be no legal recourse against someone selling seed of those varieties under a different name, I still find it morally wrong.
 

BigBonner

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University of Kentucky at one time and may still do , Develops new varieties of tobacco for seed companies .

I may check out Gold leaf for seeds . I have bought from Workman and Rickards and handed the seeds back because of the country of origin , Costa Rico , Argentina ?? Not all their seeds but most of them .

What about tobacco genetics , Do you believe they have messed with Burley varieties ? There has been questions of GMO Tobacco .
I know with modern LC seeds tobacco does not weigh like it did back in the Mid 90'S , Leaves seem light bodied and shorter .
 

JessicaNicot

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there are currently no GMO tobacco varieties on the market. they have been developed for research purposes only but are highly regulated and have never been released to the public.

I don't know a lot about burley breeding but the changes could be due to the incorporation of resistance genes. burley varieties generally have a couple different genomic introgressions from different nicotiana species which confer disease resistance and each one comes with its own yield and/or quality penalty.

the LC designation comes from a single unstable genetic locus for nicotine conversion into nornicotine. when plants for seed production are grown out, each plant is tested for nornicotine and ones with high levels are culled from the field. the plants that remain are called "low converters", LC. all standard burley lines will eventually revert to high nornicotine conversion rates (which is bad because it is a precursor for the nastiest tobacco carcinogens) if the culling procedure is not performed. (there is an aside here for these new stable reduced converter-SRC- lines that are coming out of NCSU that do not revert to high nornicotine conversion.)
 

chillardbee

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There was one GMO baccy plant that I read about that was designed to weather droughts. I can't seem to find that article anymore but I did come across this-

http://www.sciencedaily.com/releases/2014/04/140414092002.htm

Modified to increase the sugar and starch production to be used in the bio-fuel industry. I wonder how that might effect flavour of the baccy if it were used smoking tobacco?

Then there there's the injecting of the ebola virus to produce protiens that can be made into a serum to fight the virus. It was tested on two people so far back in august but I haven't seen an update on that.
 

ArizonaDave

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Then there there's the injecting of the ebola virus to produce protiens that can be made into a serum to fight the virus. It was tested on two people so far back in august but I haven't seen an update on that.

Actually I believe there's some info on that on another ebola thread, and I believe it was a rustica variety. I didn't hear about the injecting of ebola though, but then again I wish I had read the whole thing now.
 

JessicaNicot

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Actually I believe there's some info on that on another ebola thread, and I believe it was a rustica variety. I didn't hear about the injecting of ebola though, but then again I wish I had read the whole thing now.

Genes coding for specific antibodies/proteins were transformed into Nicotiana benthamiana, probably with transient expression (which is where you just get the DNA into the cells--much like a virus--rather than permanent insertion into the genome).

Tobacco is a model organism for plant transformation so researchers have generated all sorts of gmo tobacco to make sure their new genes work like they intended. but specific projects that people have looked at in tobacco include tweeking genes for biofuels or bioremediation (which is where plants are used to extract compounds like toxic heavy metals from the soil); gmo tobacco with resistance to certain viruses (which is relatively easy to do, and the mechanism in plants--known as RNA interference--was used to discover similar pathways in humans that have been used to develop novel drug therapies for genetic conditions); textiles/biopharma (a really neat purple xanthi plant was developed here at ncsu in another program by insertion of a gene encoding for anthocyanin--red/pink pigment); my boss developed a neat little tool for accelerated backcross breeding of tobacco by inserting a copy of a flower induction gene which turns on as soon as the plants germinate so you get tiny tobacco plants about 6" high with viable flowers.
 

leverhead

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... my boss developed a neat little tool for accelerated backcross breeding of tobacco by inserting a copy of a flower induction gene which turns on as soon as the plants germinate so you get tiny tobacco plants about 6" high with viable flowers.

Does this need to be done for each generation?
 

JessicaNicot

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Does this need to be done for each generation?

so say you make a cross of AWESOME by K326 in order to transfer some desired resistance gene from AWESOME to a superior yielding K326 background. We keep a line called K326 FT which is heterozygous for the early flowering transgene. You take your AWESOME pollen and cross it onto K326 FT and get a couple capsules of seed. when you germinate that seed you get plants that are genetically 50% AWESOME/50% K326 FT. Assuming AWESOME was homozygous for the resistance gene, all progeny in the first generation are heterozygous for that trait of interest and segregating for FT. So select some FT plants and pollinate them with regular K326 pollen (you can't self FT plants or cross FT by FT because you risk fixing the transgene, and that's bad). You continue backcrossing this way (to regular K326) selecting for FT and your gene of interest for several generations and then when you believe the line is sufficiently inbred to be uniform, rather than selecting the plants with FT you select normal phenotype (collect seed from it and put it into trials, etc). It's VERY fast. In the few years we have been using it we have backcrossed so many genes into so many cultivars that it would have been an impossible dream only 10 yrs ago.
 

leverhead

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so say you make a cross of AWESOME by K326...

That had me chuckling through 3 rereads. I think I have it now, thank you. So the real trick is to have both K326 and K326 FT on hand. You breed in K326 FT once to fast forward and then breed in K326 to give you a way out of the loop. I'm guessing that you use genetic testing to "see" what you're doing. :) Maybe it should be K326 >>l. Cool tools!
 

JessicaNicot

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you know what's funny?

FOUR YEARS later and I'm back here to steal my own pics! hahaha

idk what happened to the originals, but I needed process photos of trimming, bagging, harvesting, etc for a presentation that I'm giving in a couple weeks. I'm going to do a presentation in CUBA! Can you say all inclusive sea side resort?! =) Sorry fellas, I can only bring back $100 of tobacco products and alcohol, and that's pretty much all for my brother.
 
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