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Workhorse's 2013 bacca patch

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JessicaNicot

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Tell us more about your Masters Project, maybe start a new thread. I'll bet there are quite a few people here interested in it.

i can do better than that. if you're REALLY interested and have the time to download a gigantic pdf file, you can read my thesis. most universities are now open-source hosting their theses/disertations online. its about genetic and phenotypic diversity of N. rustica, as well as reactions to different diseases (and nearly half the pages are data output from statistical programs that arent worth looking at). http://www.lib.ncsu.edu/resolver/1840.16/2541
 

JessicaNicot

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By lowering the alkoloid content of the plant we lower the resistance to insects?

i think thats generally true, but not always. nicotine is a natural insecticide and people have looked at it for organic systems for a long time. however, there are some varieties of tobacco that (i think) have low alkaloid (the broader name for nicotine and similar molecules) content but are also reistant to horn worms, etc. these lines have unusual trichomes (the fuzzy leaf hairs) or they dont have trichomes at all. i tried to look at GRIN for alkloid data on these lines (PIs 511808, 511809, 537447) but they apparently were not included in those studies. i know that they generated some genetically modified tobacco lines that did not make alkaloids and i heard they were torn up by bugs.
 

JessicaNicot

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Wow, I just wish I could get a hornworm resistant bacca :)

"CU-2 (PI 511808) was derived from a 1980 cross of Coker 347 (PI 552462) X TI 1112 (PI 124166). Cu-2 was resistant to tobacco budworm, hornworm, and aphid in the field, and sustained 83 and 33% less budworm and hornworm damage, respectively, than NC 2326 (PI 552453). Aphid infestations were 85% less on CU-2 than on NC 2326. Cu-2 has leaf trichomes without glandular heads or exudates. CU-2 also was a nicotine converter, and 80% of the total alkaloid level (1.41% dry wt of cured leaf) was nornicotine." (Johnson. 1988. Crop Science 28:383)


"1-35 (PI 551301) is a tobacco hornworm (Manduca sexta L.) resistant cultivar. It is a doubled haploid derived from the F1 of TI 1112 (PI 124166) × Speight G-33 (PI 552490). Neither of the parents have resistance to the hornworm. The haploid from the F1 was obtained by anther culture and the chromosome number was doubled with colchicine. The doubled haploid was in the S4 generation when released in 1980." (Miles at al. 1981. Crop Science 21:802)
 

BigBonner

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"CU-2 (PI 511808) was derived from a 1980 cross of Coker 347 (PI 552462) X TI 1112 (PI 124166). Cu-2 was resistant to tobacco budworm, hornworm, and aphid in the field, and sustained 83 and 33% less budworm and hornworm damage, respectively, than NC 2326 (PI 552453). Aphid infestations were 85% less on CU-2 than on NC 2326. Cu-2 has leaf trichomes without glandular heads or exudates. CU-2 also was a nicotine converter, and 80% of the total alkaloid level (1.41% dry wt of cured leaf) was nornicotine." (Johnson. 1988. Crop Science 28:383)


"1-35 (PI 551301) is a tobacco hornworm (Manduca sexta L.) resistant cultivar. It is a doubled haploid derived from the F1 of TI 1112 (PI 124166) × Speight G-33 (PI 552490). Neither of the parents have resistance to the hornworm. The haploid from the F1 was obtained by anther culture and the chromosome number was doubled with colchicine. The doubled haploid was in the S4 generation when released in 1980." (Miles at al. 1981. Crop Science 21:802)


I see those studies were from 1988 and 1981 . From what I understand is that bugs build up a resistance to insecticides . This would make me wonder if those plants would give same results in todays mutant bug populations .I also wonder about bugs in different states . I know some have different bug problems that I have never seen .
 

Fisherman

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I read the entire thesis...
Can't say I understood all of it but is fascinating none the less and informative as well.
Especially tha parts about the difficilties in getting that first cross.
 

JessicaNicot

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I see those studies were from 1988 and 1981 . From what I understand is that bugs build up a resistance to insecticides . This would make me wonder if those plants would give same results in todays mutant bug populations .I also wonder about bugs in different states . I know some have different bug problems that I have never seen .

those are quotes from the official summary published when the varieties were registered and released. those varieties were developed in SC by clemson i believe but were never widely grown and thus i do not believe that there has been opportunity in the insect population to overcome the resistance mechanism.
 

workhorse_01

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Bigbonner I understand that could go both ways, either the worms could have surpassed the plants ability to defend itself, or the plant could be completely toxic to the worms.
 

workhorse_01

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Jessica, what are the odds of windblown cross pollination using those paint screen bags? I know I don't know how many microns the mesh is but I don't need a thousand professional bags but I do wish to keep my strains pure.
 

Fisherman

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I will take 100 or more of the Delnet bags at least and hold 100 in case anyone wants them later.
Workhorse.......... She said they used the bags mainly for keeping insects out but the delnet bags are only about 65 cents each if bought by 1000. My only concern is the possible heat inside the bags. We havent even hit the 90's yet and overdue by a month at that.
 

workhorse_01

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I'm sorry I didn't get back with you, I had my brother and his wife up from s.Fla. They're going to Darlington for the race. Maybe I'm worrying over nothing, I read somewhere that tobacco can cross-pollinate up to a mile.
 

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I'm sorry I didn't get back with you, I had my brother and his wife up from s.Fla. They're going to Darlington for the race. Maybe I'm worrying over nothing, I read somewhere that tobacco can cross-pollinate up to a mile.

That is usually given for the reason to bag your plants even if you think you're the only one around growing tobacco and you're only growing one variety. Some folks think they are safe and don't bag because they only have one variety and they don't know of anyone around them growing tobacco. That is just wishful thinking. My cousin grew some cigar varieties last year right down the road and I didn't know it until this year. So you can't just assume your plants won't get cross pollinated by someone else's plants, you need to bag. The bag just needs to keep out the pollinating insects. Jessica talks about how difficult it is for tobacco to pollinate by wind in her Grow Blog. Unlike corn, which is easily pollinated by wind.
 

JessicaNicot

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Jessica, what are the odds of windblown cross pollination using those paint screen bags? I know I don't know how many microns the mesh is but I don't need a thousand professional bags but I do wish to keep my strains pure.

the odds are extremely remote. our bags are to keep insects/worms out only. tobacco pollen isnt very mobile at all. i was trying to find a reference to share with you guys but my home computer is being a jerk.
 
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